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gelred  (Biotium)


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    Structured Review

    Biotium gelred
    Gelred, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3980 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gelred+nucleic+acid+stain/GelRed+Nucleic+Acid+Gel+Stain/10__1007_slash_s41348___026___01351___x-128-35-36
    Average 97 stars, based on 3980 article reviews
    gelred - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Staining:

    Article Title: Self-amplifying CRISPR-based one-pot ultrasensitive testing for rapid SARS-CoV-2 and its variant detection
    Article Snippet: Rapid, accessible molecular tests that can resolve viral variants remain a critical unmet need.. We report a one‐tube clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 13a (Cas13a) assay that couples target recognition to a T7‐promoter–driven self-amplifying loop, thereby achieving exponential fluorescence amplification at a single temperature (37 °C) within 40 min.. Without separate pre‐ amplification, the assay detects severe acute respiratory syndrome coronavirus 2 (SARS‐CoV ‐2) open-reading frame 1a (ORF1a), nucleocapsid (N), spike (S), and envelope (E) RNAs with limits of detection (LoDs) of 0.32–0.96 copies μL−1, corresponding to attomolar level sensitivity.

    Article Title: Detection of Cariogenic Bacteria in Pediatric Carious Dentin: Comparative Analysis of Culture and PCR Methods
    Article Snippet: .. Following amplification, the PCR products were separated through 2% agarose gel electrophoresis using GelRed® nucleic acid stain (Biotium, Inc., Fremont, CA, USA) and analyzed semi-quantitatively with the documentation system InGenius 3 (Syngene, Cambridge, UK). .. Molecular weight markers were used to determine the resulting PCR products: 100–1000 bp DNA Ladder (Meridian Bioscience, Cincinnati, OH, USA).

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Article Title: Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies
    Article Snippet: DNA Gel Loading Dye (6X) , Thermo Fisher , Cat#R0611. .. GelRed Nucleic Acid Stain, 10,000x , Biotium , Cat#41003-1. .. AMPure XP, 60 mL , Beckman Coulter , Cat#A63881.

    Article Title: Oral Mucositis in Oncopediatric Patients: MTX and MMP-1, MMP-8, MMP-13 Gene Polymorphisms.
    Article Snippet: A 10 μL aliquot of PCR products was then digested with 1 unit of specific enzyme (Restriction Enzymes Thermo Scientific—Fermentas Life Science—St. .. Lithuania, UE) overnight and was electrophoresed on a 5% agarose gel or 10% polyacrylamide gel and stained by GelRed Nucleic Acid Stain (Biotium Inc.—St. ..

    Article Title: PCR-Based Screening of Resistance Genes in Multidrug-Resistant Nosocomial Bacterial Isolates from Hospitalized Patients: Implications for Microbiology and Immunology Aspects
    Article Snippet: Nuclease-free water (Thermo Fisher; Cat. No. R0581) was used for reagent preparation. .. Electrophoresis was performed using agarose (Invitrogen, USA; Cat. No. 16500 - 500), GelRed nucleic acid stain (Biotium, USA; Cat. No. 41003), and a 100-bp DNA ladder (Thermo Fisher; Cat. No. SM0241); gels were visualized under UV transillumination. .. Instruments included an incubator (Memmert, Germany; Cat. No. IN55), a Class II biosafety cabinet (ESCO, Singapore), an autoclave (Sanyo, Japan), a laminar flow hood (Nuaire, USA), a NanoDrop 2000 spectrophotometer (Thermo Fisher), an Eppendorf thermal cycler (Eppendorf AG, Germany; Cat. No. 6331000), a gel electrophoresis apparatus (Bio-Rad, USA; Cat. No. 170 - 4486), a UV transilluminator, and a gel documentation system (Bio-Rad; Cat. No. 170 - 8195).

    Article Title: Selective antitumor and apoptosis-inducing effects of the Src inhibitor PP1 in human tongue squamous cell carcinoma cells
    Article Snippet: .. Intact or fragmented DNA was extracted and subjected to electrophoresis at 50 V on a 1.8% agarose gel containing GelRed Nucleic Acid Stain (cat. no. 41003; Biotium, Inc.) for 40 min. .. Subsequently, the intact or fragmented DNA was visualized and captured under UV illumination post-staining with ethidium bromide (0.1 μg/ml) using a Gel documentation system (Gel Doc-XR; Bio-Rad Laboratories, Inc.).

    Article Title: Formulation and biological evaluation of a Perilla frutescens seed oil nanoemulsion: A novel therapeutic agent for alleviating gut-liver axis inflammation.
    Article Snippet: Background: The gut-liver axis is integral to the pathophysiology of chronic liver inflammation and related metabolic inflammatory disease.. Although Perilla frutescens seed oil (PFSO) possesses anti-inflammatory properties, its clinical application is limited by its poor stability and bioavailability.. This study aimed to develop a PFSO-loaded nanoemulsion (PFSO-NE) to enhance its therapeutic efficacy.

    Amplification:

    Article Title: Detection of Cariogenic Bacteria in Pediatric Carious Dentin: Comparative Analysis of Culture and PCR Methods
    Article Snippet: .. Following amplification, the PCR products were separated through 2% agarose gel electrophoresis using GelRed® nucleic acid stain (Biotium, Inc., Fremont, CA, USA) and analyzed semi-quantitatively with the documentation system InGenius 3 (Syngene, Cambridge, UK). .. Molecular weight markers were used to determine the resulting PCR products: 100–1000 bp DNA Ladder (Meridian Bioscience, Cincinnati, OH, USA).

    Polymerase Chain Reaction:

    Article Title: Detection of Cariogenic Bacteria in Pediatric Carious Dentin: Comparative Analysis of Culture and PCR Methods
    Article Snippet: .. Following amplification, the PCR products were separated through 2% agarose gel electrophoresis using GelRed® nucleic acid stain (Biotium, Inc., Fremont, CA, USA) and analyzed semi-quantitatively with the documentation system InGenius 3 (Syngene, Cambridge, UK). .. Molecular weight markers were used to determine the resulting PCR products: 100–1000 bp DNA Ladder (Meridian Bioscience, Cincinnati, OH, USA).

    Article Title: Formulation and biological evaluation of a Perilla frutescens seed oil nanoemulsion: A novel therapeutic agent for alleviating gut-liver axis inflammation.
    Article Snippet: Background: The gut-liver axis is integral to the pathophysiology of chronic liver inflammation and related metabolic inflammatory disease.. Although Perilla frutescens seed oil (PFSO) possesses anti-inflammatory properties, its clinical application is limited by its poor stability and bioavailability.. This study aimed to develop a PFSO-loaded nanoemulsion (PFSO-NE) to enhance its therapeutic efficacy.

    Agarose Gel Electrophoresis:

    Article Title: Detection of Cariogenic Bacteria in Pediatric Carious Dentin: Comparative Analysis of Culture and PCR Methods
    Article Snippet: .. Following amplification, the PCR products were separated through 2% agarose gel electrophoresis using GelRed® nucleic acid stain (Biotium, Inc., Fremont, CA, USA) and analyzed semi-quantitatively with the documentation system InGenius 3 (Syngene, Cambridge, UK). .. Molecular weight markers were used to determine the resulting PCR products: 100–1000 bp DNA Ladder (Meridian Bioscience, Cincinnati, OH, USA).

    Article Title: Oral Mucositis in Oncopediatric Patients: MTX and MMP-1, MMP-8, MMP-13 Gene Polymorphisms.
    Article Snippet: A 10 μL aliquot of PCR products was then digested with 1 unit of specific enzyme (Restriction Enzymes Thermo Scientific—Fermentas Life Science—St. .. Lithuania, UE) overnight and was electrophoresed on a 5% agarose gel or 10% polyacrylamide gel and stained by GelRed Nucleic Acid Stain (Biotium Inc.—St. ..

    Article Title: Selective antitumor and apoptosis-inducing effects of the Src inhibitor PP1 in human tongue squamous cell carcinoma cells
    Article Snippet: .. Intact or fragmented DNA was extracted and subjected to electrophoresis at 50 V on a 1.8% agarose gel containing GelRed Nucleic Acid Stain (cat. no. 41003; Biotium, Inc.) for 40 min. .. Subsequently, the intact or fragmented DNA was visualized and captured under UV illumination post-staining with ethidium bromide (0.1 μg/ml) using a Gel documentation system (Gel Doc-XR; Bio-Rad Laboratories, Inc.).

    Article Title: Formulation and biological evaluation of a Perilla frutescens seed oil nanoemulsion: A novel therapeutic agent for alleviating gut-liver axis inflammation.
    Article Snippet: Background: The gut-liver axis is integral to the pathophysiology of chronic liver inflammation and related metabolic inflammatory disease.. Although Perilla frutescens seed oil (PFSO) possesses anti-inflammatory properties, its clinical application is limited by its poor stability and bioavailability.. This study aimed to develop a PFSO-loaded nanoemulsion (PFSO-NE) to enhance its therapeutic efficacy.

    Fluorescence:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Microscopy:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Single Cell Gel Electrophoresis:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Software:

    Article Title: Glucose coated FeO@Fe 3 O 4 nanoparticles show tunable catalytic reactivity and safety in a 3D hepatic in vitro model.
    Article Snippet: .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA). .. Slides were stained with GelRed nucleic acid stain (Biotium, USA) following the manufacturer’s instructions, and comet scoring was carried out using a fluorescence microscope and Comet Assay IV software (Instem, Philadelphia, USA).

    Electrophoresis:

    Article Title: PCR-Based Screening of Resistance Genes in Multidrug-Resistant Nosocomial Bacterial Isolates from Hospitalized Patients: Implications for Microbiology and Immunology Aspects
    Article Snippet: Nuclease-free water (Thermo Fisher; Cat. No. R0581) was used for reagent preparation. .. Electrophoresis was performed using agarose (Invitrogen, USA; Cat. No. 16500 - 500), GelRed nucleic acid stain (Biotium, USA; Cat. No. 41003), and a 100-bp DNA ladder (Thermo Fisher; Cat. No. SM0241); gels were visualized under UV transillumination. .. Instruments included an incubator (Memmert, Germany; Cat. No. IN55), a Class II biosafety cabinet (ESCO, Singapore), an autoclave (Sanyo, Japan), a laminar flow hood (Nuaire, USA), a NanoDrop 2000 spectrophotometer (Thermo Fisher), an Eppendorf thermal cycler (Eppendorf AG, Germany; Cat. No. 6331000), a gel electrophoresis apparatus (Bio-Rad, USA; Cat. No. 170 - 4486), a UV transilluminator, and a gel documentation system (Bio-Rad; Cat. No. 170 - 8195).

    Article Title: Selective antitumor and apoptosis-inducing effects of the Src inhibitor PP1 in human tongue squamous cell carcinoma cells
    Article Snippet: .. Intact or fragmented DNA was extracted and subjected to electrophoresis at 50 V on a 1.8% agarose gel containing GelRed Nucleic Acid Stain (cat. no. 41003; Biotium, Inc.) for 40 min. .. Subsequently, the intact or fragmented DNA was visualized and captured under UV illumination post-staining with ethidium bromide (0.1 μg/ml) using a Gel documentation system (Gel Doc-XR; Bio-Rad Laboratories, Inc.).

    Imaging:

    Article Title: Formulation and biological evaluation of a Perilla frutescens seed oil nanoemulsion: A novel therapeutic agent for alleviating gut-liver axis inflammation.
    Article Snippet: Background: The gut-liver axis is integral to the pathophysiology of chronic liver inflammation and related metabolic inflammatory disease.. Although Perilla frutescens seed oil (PFSO) possesses anti-inflammatory properties, its clinical application is limited by its poor stability and bioavailability.. This study aimed to develop a PFSO-loaded nanoemulsion (PFSO-NE) to enhance its therapeutic efficacy.



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